| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human pro Caspase 9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GAH-3 is a rabbit monoclonal antibody that recognizes caspase-9 (gene CASP9) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 46.3 kDa, and the supplier reports an observed band at about 46 kDa.
Caspase-9 is the initiator caspase of the intrinsic, mitochondrial apoptosis pathway. When cytochrome c is released from mitochondria, it binds Apaf-1 to form the apoptosome, which activates caspase-9; caspase-9 then cleaves and activates caspase-3 and caspase-7. It is a standard marker in studies of DNA damage-induced and stress-induced apoptosis.
| Target | Caspase-9 (gene CASP9; UniProt P55211, human) |
|---|---|
| Clone | GAH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.3 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with confirmed staining (IHC): human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human pro Caspase 9.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Özlem Kaplan et al. (2024) Development of recombinant protein-based nanoparticle systems for inducing tumor cell apoptosis: In vitro evaluation of their cytotoxic and apoptotic effects on cancer cells. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2024.105565
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Kaplan Özlem (2023) Synergistic induction of apoptosis in liver cancer cells: exploring the combined potential of doxorubicin and XL-888. Medical Oncology. 10.1007/s12032-023-02181-9
Qun Li et al. (2019) SOX2-induced upregulation of lncRNA LINC01510 promotes papillary thyroid carcinoma progression by modulating miR-335/SHH and activating Hedgehog pathway. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2019.09.138
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Nazan Gökşen Tosun et al. (2022) Green synthesized silver nanoparticles with mushroom extracts of Paxina leucomelas and Rhizopogon luteolus induce ROS-Induced intrinsic apoptotic pathway in cancer cells. Inorganic and Nano-Metal Chemistry. 10.1080/24701556.2022.2081200
