Anti-Prohibitin PHB Rabbit Monoclonal Antibody

Overview

This product is an anti-PHB antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DIF-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Prohibitin PHB Rabbit Monoclonal Antibody catalog # M01178. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: PHB (Prohibitin).
• Antibody format: Monoclonal; clone DIF-16; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

PHB (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. . Reported cellular localization context: Mitochondrion inner membrane . Tissue expression notes (as provided): Widely expressed in different tissues.

Research relevance and current trends

• Research context keywords from the source record include: Apoptosis,Cancer,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Cell Death,Co-Factors,Epigenetics and Nuclear Signaling,Metabolism,Metabolism Processes,Mitochondria,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Organelles,Pathways and Processes,Subcellular Markers,Tags & Cell Markers,Transcription.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate PHB antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PHB expression by Western blot in cell or tissue lysates, Detect PHB in FFPE tissue sections by immunohistochemistry, Localize PHB by immunofluorescence/immunocytochemistry in cultured cells, Quantify PHB-positive cells by flow cytometry in single-cell suspensions, Enrich PHB by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 30 kDa; calculated MW: 29804 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 30 kDa
• Cellular localization (provided): Mitochondrion inner membrane .
• Tissue details (provided): Widely expressed in different tissues.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.