Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®

Overview

Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® is an antibody for Sftpc detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Sftpc (Bifunctional epoxide hydrolase 2); UniProt: P21841
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 21 kDa, calculated 62616 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® catalog # A02001.

Biological background

Biological context: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. .

Expression and localization notes: cellular localization: Cytoplasm. Peroxisome., tissue context: Ubiquitous. A high level expression is seen in secretory tissues..

Common research applications

• Western blotting (WB): Compare Sftpc levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Sftpc in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Sftpc-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: SFTPC (Surfactant pulmonary -associated protein C), also known as SFTP2, SPC, PSP-C or PULMONARY SURFACTANT PROTEIN SP5, is a protein that in humans is encoded by the SFTPC gene. It is a membrane protein which manufactures surfactant. Glasser et al. (1988) assigned the SFTPC gene, symbolized SFTP2 by the Human Gene Mapping nomenclature committee, to chromosome 8 by hybridization to somatic cell hybrid DNA. Wood et al. (1994) identified 4 cosmids at the SFTP2 locus by cDNA hybridization. Using a polymorphic CA repeat in the SFTP2 gene and linkage analysis in 8 CEPH families, they located the gene close to D8S298; maximum lod = 19.3 at theta = 0.01. They regionalized the gene to 8p21 by fluorescence in situ hybridization. Moore et al. (1992) showed that the corresponding mouse gene, Sftp2, maps to chromosome 14.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm. Peroxisome.
• Tissue details: Ubiquitous. A high level expression is seen in secretory tissues.
• Research category: Cancer Metabolism,Cardiovascular,Cell Biology,Cholesterol Metabolism,Drug Metabolism,Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Redox Metabolism,Signal Transduction,Vasculature

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.