| Field | Specification |
|---|---|
| Target | |
| Alternative names | Surfactant protein C; SP-C; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5; Sftpc; Sftp2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Prosurfactant Protein C/SFTPC recombinant protein (Position: S98-I193). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects surfactant protein C (gene Sftpc) in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 21.1 kDa, and the supplier reports an observed band at about 21 kDa.
Surfactant protein C (SP-C, Sftpc) is a small, highly hydrophobic lung surfactant protein made only by alveolar type II epithelial cells. It helps the surfactant film lower surface tension and keep alveoli open, and in mouse it is widely used as a marker of type II cells in lung development and injury research.
| Target | Surfactant protein C (gene Sftpc; UniProt P21841, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 21.1 kDa |
| Observed MW | 21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Prosurfactant Protein C/SFTPC fragment (Ser98–Ile193), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Wenqi She et al. (2023) Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips. Lab on a Chip. 10.1039/D3LC00807J
Lawei Yang et al. (2020) DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2020.111283
Hui-Ling Jiang et al. (2024) CGRP is essential for protection against alveolar epithelial cell necroptosis by activating the AMPK/L-OPA1 signaling pathway during acute lung injury. Journal of Cellular Physiology. 10.1002/jcp.31169
Chong Shen et al. (2023) A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane. Biotechnology and Bioengineering. 10.1002/bit.28426
