| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein phosphatase non-receptor type 12; PTP-PEST; Protein-tyrosine phosphatase G1; PTPG1; PTPN12 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PTPN12 recombinant protein (Position: A276-D311). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tyrosine-protein phosphatase non-receptor type 12 (gene PTPN12) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 88.1 kDa, and the supplier reports an observed band at about 110 kDa.
PTP-PEST (PTPN12) is a cytoplasmic tyrosine phosphatase that dephosphorylates kinases such as ERBB2 (HER2) and PYK2, as well as focal adhesion proteins. By limiting these signals it influences cell migration and growth factor signaling, and it is studied as a regulator of cell motility and in breast cancer.
| Target | Tyrosine-protein phosphatase non-receptor type 12 (gene PTPN12; UniProt Q05209, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.1 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HL-60 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human PTPN12 fragment (Ala276–Asp311), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein phosphatase non-receptor type 12 in the cytoplasm, cell junction and cell projection. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
