| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein phosphatase non-receptor type 13; Fas-associated protein-tyrosine phosphatase 1; FAP-1; PTP-BAS; Protein-tyrosine phosphatase 1E; PTP-E1; hPTPE1; Protein-tyrosine phosphatase PTPL1; PTPN13; PNP1; PTP1E; PTPL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PTPN13 recombinant protein (Position: E1795-D2046). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tyrosine-protein phosphatase non-receptor type 13 (gene PTPN13) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 276.9 kDa, and the supplier reports an observed band at about 270 kDa.
PTPN13 (FAP-1, PTP-BAS) is a large cytoplasmic tyrosine phosphatase with multiple PDZ domains. It binds the death receptor FAS and restrains FAS-induced apoptosis, and it can also dampen NGFR-mediated death signals and PI3K signaling.
| Target | Tyrosine-protein phosphatase non-receptor type 13 (gene PTPN13; UniProt Q12923, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 276.9 kDa |
| Observed MW | 270 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Recombinant human PTPN13 fragment (Glu1795–Asp2046), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein phosphatase non-receptor type 13 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Expressed in keratinocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
