| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peroxiredoxin-6;1.11.1.15;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein;Acidic calcium-independent phospholipase A2;aiPLA2;3.1.1.-;Antioxidant protein 2;Liver 2D page spot 40;Non-selenium glutathione peroxidase;NSGPx;1.11.1.9;Red blood cells page spot 12;PRDX6;AOP2, KIAA0106; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human RAP1A + RAP1B |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RAP1A/B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22R89; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-RAP1A + RAP1B Rabbit Monoclonal Antibody catalog # M01848. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: RAP1A/B (Peroxiredoxin-6).
- Antibody format: Monoclonal; clone 22R89; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RAP1A/B (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in redox regulation of the cell. Can reduce H (2)O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Reported cellular localization context: Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles. . Tissue expression notes (as provided): Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. .
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Heart Disease,Lipid Metabolism,Metabolism,Mitochondrial Markers,Mitochondrial Metabolism,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate RAP1A/B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RAP1A/B expression by Western blot in cell or tissue lysates, Quantify RAP1A/B-positive cells by flow cytometry in single-cell suspensions, Enrich RAP1A/B by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 21 kDa; calculated MW: 25035 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 21 kDa
- Cellular localization (provided): Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles. .
- Tissue details (provided): Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
