| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras GTPase-activating protein 1; GAP; GTPase-activating protein; RasGAP; Ras p21 protein activator; p120GAP; RASA1; RASA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RASA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27R42 is a rabbit monoclonal antibody that recognizes ras GTPase-activating protein 1 (gene RASA1) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 116.4 kDa, and the supplier reports an observed band at about 140 kDa.
RASA1 (p120 RasGAP) is a GTPase-activating protein that speeds up GTP hydrolysis by Ras, returning Ras to its inactive GDP-bound form and ending its signaling. It is a key negative regulator of Ras–MAPK signaling, and RASA1 mutations cause capillary malformation–arteriovenous malformation, so it is studied in vascular development and growth factor signaling.
| Target | Ras GTPase-activating protein 1 (gene RASA1; UniProt P20936, human) |
|---|---|
| Clone | 27R42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.4 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): rat brain cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RASA1.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places ras GTPase-activating protein 1 in the cytoplasm. Tissue expression noted by UniProt (human): In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
