Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4)

Overview

Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4) is an antibody for RBPJ detection raised in Mouse (Monoclonal, clone Clone: 6G4, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: RBPJ (zeta chain of T cell receptor associated protein kinase 70kDa); UniProt: Q06330
• Antibody format: Mouse, Monoclonal, clone Clone: 6G4, Mouse IgG2b
• Molecular weight: 56 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4) catalog # M00767-1.

Biological background

Biological context: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Expression and localization notes: cellular localization: Cell membrane. Cytoplasm. Membrane., tissue context: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells..

Common research applications

• Western blotting (WB): Compare RBPJ levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of RBPJ in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify RBPJ-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene. It is mapped to 4p15.2. The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Cytoplasm. Membrane.
• Tissue details: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
• Research category: Diabetes-associated,Heart Disease,Metabolism,Protein Phosphorylation,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.