| Field | Specification |
|---|---|
| Target | |
| Alternative names | Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30; RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RBPJK/RBPJ recombinant protein (Position: K41-Q467). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6G4 is a mouse monoclonal antibody that recognizes recombining binding protein suppressor of hairless (gene RBPJ) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 55.6 kDa, and the supplier reports an observed band at about 56 kDa.
RBPJ (also called CSL or RBP-Jκ) is the DNA-binding transcription factor at the heart of Notch signaling. Alone it represses Notch target genes by recruiting corepressor complexes, but when the Notch intracellular domain enters the nucleus it forms an activating complex that turns these genes on. RBPJ is therefore central to studies of cell fate decisions, development, stem cells and immunity.
| Target | Recombining binding protein suppressor of hairless (gene RBPJ; UniProt Q06330, human) |
|---|---|
| Clone | 6G4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 55.6 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
Samples with a confirmed band (WB): human HEK293 cells, human HepG2 cells, human HeLa cells, human MCF-7 cells, human U87 cells, human HL-60 cells, rat NRK cells, mouse smooth muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells, U937 cells.
Immunogen
Recombinant human RBPJK/RBPJ fragment (Lys41–Gln467), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places recombining binding protein suppressor of hairless in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
