| Field | Specification |
|---|---|
| Target | |
| Alternative names | Resistin;Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RETN;FIZZ3, HXCP1, RSTN;UNQ407/PRO1199; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Resistin recombinant protein (Position: K19-P108). Human Resistin shares 58.6% amino acid (aa) sequence identity with mouse Resistin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets resistin (gene RETN) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 11.4 kDa, and the supplier reports an observed band at about 11 kDa.
Resistin (RETN) is a small cysteine-rich secreted protein. In mice it is made by adipocytes and was named for its ability to cause insulin resistance, whereas in humans it comes mainly from monocytes and macrophages and is tied more closely to inflammation. It is measured in studies of obesity, insulin resistance and inflammatory disease.
| Target | Resistin (gene RETN; UniProt Q9HD89, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 11.4 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Resistin fragment (Lys19–Pro108), expressed in E. coli. Sequence identity with the mouse ortholog: 58.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes resistin as a secreted protein. Tissue expression noted by UniProt (human): Expressed in white adipose tissue (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Helber da Maia Valenca et al. (2024) Therapeutic Potential of Dimethyl Fumarate for the Treatment of High-Fat/High-Sucrose Diet-Induced Obesity. Antioxidants. 10.3390/antiox13121496
Xiao-Lei Yao et al. (2010) Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye. International Journal of Ophthalmology. 10.3980/j.issn.2222-3959.2010.02.05
