| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribonuclease inhibitor;Placental ribonuclease inhibitor;Placental RNase inhibitor;Ribonuclease/angiogenin inhibitor 1;RAI;RNH1;PRI, RNH; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
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Overview
This antibody is intended for detection of RNH1 (Ribonuclease inhibitor) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® catalog # PB9885. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
- Molecular weight context: reported MW: 50 kDa; calculated MW: 49973 MW
- Reactivity: Human,Monkey,Mouse,Rat
- Applications: Flow Cytometry, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Ribonuclease inhibitor; Ribonuclease inhibitor. Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo. Functional note: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis. . Reported localization: Cytoplasm. Expression/tissue context: Widely expressed; high expression found in skeletal muscle. .
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how RNH1 (Ribonuclease inhibitor) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how RNH1 (Ribonuclease inhibitor) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA: Researchers commonly examine how RNH1 (Ribonuclease inhibitor) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative RNH1 (Ribonuclease inhibitor) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of RNH1 (Ribonuclease inhibitor) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
