Anti-RING1 Rabbit Monoclonal Antibody

Overview

This product is an anti-RING1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22R33; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-RING1 Rabbit Monoclonal Antibody catalog # M01824-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Key elements and design rationale

• Target: RING1 (Midkine).
• Antibody format: Monoclonal; clone 22R33; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

RING1 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity). . Reported cellular localization context: Secreted. Tissue expression notes (as provided): Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions. .

Research relevance and current trends

• Research context keywords from the source record include: Growth and Development,Neurogenesis,Neurology Process,Neuroscience.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.

Workflow ideas (metafield): Validate RING1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RING1 expression by Western blot in cell or tissue lysates, Detect RING1 in FFPE tissue sections by immunohistochemistry, Localize RING1 by immunofluorescence/immunocytochemistry in cultured cells

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 50 kDa; calculated MW: 15585 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 50 kDa
• Cellular localization (provided): Secreted.
• Tissue details (provided): Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.