| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Endothelin-converting enzyme 1;ECE-1;3.4.24.71;ECE1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse RNase L/Rnasel recombinant protein (Position: E24-S735). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-RNase L/Rnasel Antibody Picoband® is an antibody for Rnasel detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Rnasel (Endothelin-converting enzyme 1); UniProt: Q05921
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 83 kDa, calculated 87164 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-RNase L/Rnasel Antibody Picoband® catalog # A02521-1.
Biological background
Biological context: Converts big endothelin-1 to endothelin-1. .
Expression and localization notes: cellular localization: Cell membrane; Single-pass type II membrane protein., tissue context: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. ..
Common research applications
- Western blotting (WB): Compare Rnasel levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Rnasel in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Rnasel-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane; Single-pass type II membrane protein.
- Tissue details: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. .
- Research category: Cancer,Cancer Metabolism,Cardiovascular,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Signal Transduction,Vasculature
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
