| Field | Specification |
|---|---|
| Target | |
| Alternative names | 2-5A-dependent ribonuclease; 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; RNase L; Rnasel; Rns4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse RNase L/Rnasel recombinant protein (Position: E24-S735). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 2-5A-dependent ribonuclease (gene Rnasel) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 83.3 kDa, and the supplier reports an observed band at about 83 kDa.
RNase L (2-5A-dependent ribonuclease) is an interferon-induced endoribonuclease of innate antiviral defense. It is switched on by 2′,5′-oligoadenylates made by OAS enzymes after sensing viral double-stranded RNA, then cleaves viral and cellular single-stranded RNA, including rRNA, which halts protein synthesis and can trigger apoptosis.
| Target | 2-5A-dependent ribonuclease (gene Rnasel; UniProt Q05921, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 83.3 kDa |
| Observed MW | 83 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse lung tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells, NRK cells.
Immunogen
Recombinant mouse RNase L/Rnasel fragment (Glu24–Ser735), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places 2-5A-dependent ribonuclease in the cytoplasm and mitochondrion. Tissue expression noted by UniProt (mouse): Expressed in spleen, thymus, lung, testis, kidney, liver and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
