| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-A4;Calvasculin;Metastasin;Placental calcium-binding protein;Protein Mts1;S100 calcium-binding protein A4;S100A4;CAPL, MTS1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human S100A4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GFG-19 is a rabbit monoclonal antibody that recognizes protein S100-A4 (gene S100A4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 11.7 kDa, and the supplier reports an observed band at about 12 kDa.
S100A4 (also called metastasin or FSP1) is a calcium-binding protein that binds non-muscle myosin IIA and loosens its filaments, increasing cell motility and invasiveness. It also affects p53 activity and acts outside cells to stimulate cytokine release, and it is studied in metastasis, fibrosis and inflammation.
| Target | Protein S100-A4 (gene S100A4; UniProt P26447, human) |
|---|---|
| Clone | GFG-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.7 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:100 |
Samples with confirmed staining (IHC): human thyroid cancer tissue, human esophageal carcinoma tissue, rat stomach tissue, human lung adenocarcinoma tissue, human colon carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human S100A4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes protein S100-A4 as secreted and also located in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bo Zhang et al. (2019) Dihydroartemisinin attenuates renal fibrosis through regulation of fibroblast proliferation and differentiation. Life Sciences. 10.1016/j.lfs.2019.03.020
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Hongfei Qi et al. (2026) Tanshinone ⅡA alleviates endothelial-to-mesenchymal transition by regulating the TGF-β/Smad pathway. Tissue & Cell. 10.1016/j.tice.2026.103416
