| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Phospholipid scramblase 1; PL scramblase 1; Ca (2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b; PLSCR1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Scramblase 1/PLSCR1 Antibody Picoband® is an antibody for PLSCR1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PLSCR1 (phospholipid scramblase 1); UniProt: O15162; NCBI Gene: 5359
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 35 kDa, calculated 36020 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Scramblase 1/PLSCR1 Antibody Picoband® catalog # A02995-2.
Biological background
Biological context: May mediate accelerated ATP-independent biional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
Expression and localization notes: cellular localization: Nucleus. Plasma membrane. Cell membrane. Single-pass type II membrane protein. Membrane. Lipid-anchor. Cytoplasmic side. Cytoplasm. Perinuclear region., tissue context: Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle..
Common research applications
- Western blotting (WB): Compare PLSCR1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PLSCR1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify PLSCR1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Phospholipid scramblase 1 is a protein that in human is encoded by the PLSCR1 gene. This gene is mapped to 3q24.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Plasma membrane. Cell membrane. Single-pass type II membrane protein. Membrane. Lipid-anchor. Cytoplasmic side. Cytoplasm. Perinuclear region.
- Tissue details: Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
