| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromal cell-derived factor 1;SDF-1;hSDF-1;C-X-C motif chemokine 12;Intercrine reduced in hepatomas;IRH;hIRH;Pre-B cell growth-stimulating factor;PBSF;SDF-1-beta (3-72);SDF-1-alpha (3-67);CXCL12;SDF1, SDF1A, SDF1B; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SDF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFO-3 is a rabbit monoclonal antibody that recognizes stromal cell-derived factor 1 (gene CXCL12) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 10.7 kDa, and the supplier reports an observed band at about 11 kDa.
CXCL12 (SDF-1) is a chemokine that binds the receptors CXCR4 and ACKR3. It retains hematopoietic stem cells in the bone marrow niche, guides lymphocyte and monocyte migration, and directs cell movement during embryonic development, including neural, vascular and germ cell migration. It is also studied in cancer metastasis and tissue repair.
| Target | Stromal cell-derived factor 1 (gene CXCL12; UniProt P48061, human) |
|---|---|
| Clone | IFO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.7 kDa |
| Observed MW | 11 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SDF1.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes stromal cell-derived factor 1 as a secreted protein. Tissue expression noted by UniProt (human): Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
