| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1;SERCA1;SR Ca (2+)-ATPase 1;3.6.3.8;Calcium pump 1;Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca (2+) ATPase;ATP2A1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA1 ATPase, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (gene ATP2A1) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 110.3 kDa, and the supplier reports an observed band at about 110 kDa.
ATP2A1 (SERCA1) is the calcium pump of fast-twitch skeletal muscle. It uses ATP to pump Ca2+ from the cytosol back into the sarcoplasmic reticulum, allowing muscle relaxation after contraction, and its deficiency causes Brody myopathy.
| Target | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (gene ATP2A1; UniProt O14983, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 110.3 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat skeletal muscle tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human skeletal muscle tissue, rat skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Synthetic peptide from the N-terminal region of human SERCA1 ATPase. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sarcoplasmic/endoplasmic reticulum calcium ATPase 1 in the endoplasmic reticulum membrane and sarcoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Skeletal muscle, fast twitch muscle (type II) fibers. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
