| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2;SERCA2;SR Ca (2+)-ATPase 2;3.6.3.8;Calcium pump 2;Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca (2+) ATPase;ATP2A2;ATP2B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA2 ATPase, identical to the related mouse and rat sequences. |
| Molecular weight | |
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| Reconstitution | |
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| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody targets sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (gene ATP2A2) in mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 114.8 kDa, and the supplier reports an observed band at about 115 kDa.
ATP2A2 (SERCA2) pumps Ca2+ from the cytosol into the sarcoplasmic or endoplasmic reticulum using ATP. The SERCA2a isoform controls relaxation and calcium handling in heart muscle, SERCA2b is expressed in most cells, and mutations in ATP2A2 cause Darier disease of the skin.
| Target | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (gene ATP2A2; UniProt P16615, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 114.8 kDa |
| Observed MW | 115 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human SERCA2 ATPase. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places sarcoplasmic/endoplasmic reticulum calcium ATPase 2 in the endoplasmic reticulum membrane and sarcoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Feng Yu-Lai et al. (2022) MicroRNA-200c-3p Negatively Regulates ATP2A2 and Promotes the Progression of Papillary Thyroid Carcinoma. Biochemical Genetics. 10.1007/s10528-022-10184-w
Yong Guo et al. (2013) Extracellular Matrix of Mechanically Stretched Cardiac Fibroblasts Improves Viability and Metabolic Activity of Ventricular Cells. International Journal of Medical Sciences. 10.7150/ijms.6786
