| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Shank1 Antibody is an antibody targeting SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon (also reported as SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon).
- Immunogen/epitope region: Intracellular, between the SH3 and PDZ domains..
- Homology note: Mouse - identical; human - 13/15 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Specificity statement (as provided): This antibody will recognize all known rat isoforms of Shank1 with the exception of isoform 2 (see Q9WV48-2)..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Shank family proteins (shank1, shank2 and shank3) are neuronal scaffold proteins that organize a cytoskeleton-associated signaling complex at the postsynaptic density (PSD) of nearly all excitatory glutamatergic synapses in the mammalian brain1.The Shank family, including Shank1, has five conserved protein domains: 1. ankyrin repeat domain (ANK), 2. Src homology 3 (SH3) domain, 3.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PZ011.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PZ011; Negative control: BLP-PZ011.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
