| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein deacetylase sirtuin-6;3.5.1.-;Regulatory protein SIR2 homolog 6;SIR2-like protein 6;SIRT6;SIR2L6; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SIRT6 recombinant protein (Position: D14-E180). Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NAD-dependent protein deacylase sirtuin-6 (gene SIRT6) in human and mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 39.1 kDa, and the supplier reports an observed band at about 39–42 kDa.
Sirtuin 6 (SIRT6) is a chromatin-bound NAD+-dependent enzyme that deacetylates histone H3 at lysines 9 and 56 and also removes long-chain fatty acyl groups from proteins. It supports double-strand break repair and telomere maintenance and represses glycolytic and NF-κB target genes, so it is a key subject in studies of genome stability, metabolism and aging.
| Target | NAD-dependent protein deacylase sirtuin-6 (gene SIRT6; UniProt Q8N6T7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 39.1 kDa |
| Observed MW | 39–42 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human U87 cells, human A549 cells, human K562 cells, human U2OS cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse cardiac muscle tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human SIRT6 fragment (Asp14–Glu180), expressed in E. coli. Sequence identity with the mouse ortholog: 95%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places NAD-dependent protein deacylase sirtuin-6 in the nucleus, chromosome and endoplasmic reticulum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Yang et al. (2024) Energy Stress-induced CircEPB41(2) Promotes Lipogenesis in Hepatocellular Carcinoma. Cancer Research. 10.1158/0008-5472.CAN-24-1630
Jian Liu et al. (2025) A new mechanism involved in cardiovascular senescence induced by environmentally relevant dose of 16 priority-controlled PAHs. Environment International. 10.1016/j.envint.2025.109326
Miao Jinhua et al. (2023) Sirtuin 6 is a key contributor to gender differences in acute kidney injury. Cell Death Discovery. 10.1038/s41420-023-01432-y
Li Yaling et al. (2020) Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy. Frontiers in Pharmacology. 10.3389/fphar.2020.01150
Jianan Kang et al. (2026) miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2026.110844
