| Field | Specification |
|---|---|
| Target | |
| Alternative names | S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; SKP2; FBXL1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SKP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22S45 is a rabbit monoclonal antibody that recognizes S-phase kinase-associated protein 2 (gene SKP2) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 47.8 kDa; the supplier reports observed bands at 42, 48 kDa.
SKP2 is the F-box protein that gives the SCFSKP2 ubiquitin ligase its substrate specificity. Together with CKS1 it targets phosphorylated p27 (CDKN1B), as well as p21, p57 and other cell cycle regulators, for proteasomal degradation, promoting entry into S phase, and it is widely studied as an oncogenic factor.
| Target | S-phase kinase-associated protein 2 (gene SKP2; UniProt Q13309, human) |
|---|---|
| Clone | 22S45 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.8 kDa |
| Observed MW | 42, 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SKP2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places S-phase kinase-associated protein 2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fuqiang Xu et al. (2014) The oncoprotein hepatitis B X-interacting protein promotes the migration of ovarian cancer cells through the upregulation of S-phase kinase-associated protein 2 by Sp1. International Journal of Oncology. 10.3892/ijo.2014.2411
Ying Su et al. (2011) Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts. Molecular Vision. PMID: 21270971
Xiangguo Duan et al. (2017) MicroRNA-508-5p suppresses metastasis in human gastric cancer by targeting S-phase kinase‑associated protein 2. Molecular Medicine Reports. 10.3892/mmr.2017.6793
