| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody is an antibody targeting Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1 Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1 (also reported as Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1).
- Immunogen/epitope region: 4th extracellular loop.
- Homology note: Human, rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ANT-201), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Fluorescein isothiocyanate (FITC) (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Solute carrier family 11 member a1 (Slc11a1) also known as natural resistance-associated macrophage protein 1 (NRAMP1) is a proton/divalent cation antiporter, known for its role in regulating the antimicrobial activity of macrophages1. The structure of Slc11a1 includes a common and conserved hydrophobic core of 10 transmembrane domains (TM) and either one or two non-conserved, highly hydrophobic TM domains4.Slc11a1 delivers divalent cations such as Fe2+, Zn2+, and Mn2+ from the cytosol to the late endosome/lysosome acidic compartments where the Fenton reaction can use ferrous iron to generate toxic hydroxyl radicals2. Thus, providing one mechanism by which Slc11a1 could influence antimicrobial activity in macrophages.Transition of metal ions is essential and participates in numerous cellular functions such as regulation of transcription.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-F.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NT201; Negative control: RIC-001-F.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
