| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-SLC47A1 Antibody is an antibody targeting MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein (also reported as MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein).
- Immunogen/epitope region: 5th intracellular loop.
- Homology note: Rat - identical; human - no apparent homology (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Specificity statement (as provided): Will not recognize human SLC47A1..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
MATE1 (SLC47A1) and MATE2 (SLC47A2) are multidrug and toxin extrusion transporter 1 and 2 function as cationic pumps in the kidneys, liver, and intestine1. Both MATEs excrete molecules with a positive net-charge from renal tubular epithelial cells in the kidney (where they are co-expressed with organic anion- and cation-transporters; OATs and OCTs), and into the urine. In the liver, MATE1, but not MATE2, regulates the transportation of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP) - again, by cation-powered pumps2.Fairly recent X-ray crystallography has revealed that mammalian MATE1 has 13 transmembrane helices and a long cytoplasmic tail.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NT131.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NT131; Negative control: BLP-NT131.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
