| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL (Phe); SFTPB; SFTP3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Slc7a9 recombinant protein (Position: M1-E487). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Slc7a9 Antibody Picoband® is an antibody for Slc7a9 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Slc7a9 (surfactant protein B); UniProt: Q9QXA6
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 53 kDa, calculated 31203 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Slc7a9 Antibody Picoband® catalog # A03445-1.
Biological background
Biological context: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Expression and localization notes: cellular localization: Secreted, extracellular space, surface film., tissue context: Found in the synovial fluid of patients with rheumatoid arthritis. ..
Common research applications
- Western blotting (WB): Compare Slc7a9 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Slc7a9 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Slc7a9-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: b (0,+)-type amino acid transporter 1, also known as b (0,+)AT1, is a protein which in humans is encoded by the SLC7A9 gene. This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in the high-affinity and sodium-independent transport of cystine and neutral and dibasic amino acids, and appears to function in the reabsorption of cystine in the kidney tubule. Mutations in this gene cause non-type I cystinuria, a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. Alternate transcript variants, which encode the same protein, have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted, extracellular space, surface film.
- Tissue details: Found in the synovial fluid of patients with rheumatoid arthritis. .
- Research category: Cancer,Cardiovascular,Cell Biology,Cell Cycle,Cell Differentiation,Lipases,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Vasculature
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.