| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-sloβ4 (KCNMB4) Antibody is an antibody targeting Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4 (also reported as Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4).
- Immunogen/epitope region: Intracellular, N-terminal domain.
- Homology note: Mouse, human - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
sloβ4 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel, KCa1.1. The family includes four members with a shared topology: two transmembrane domains, short intracellular N- and C-termini and a large extracellular region.The four members of the family have a distinct tissue distribution with sloβ4 expressed almost exclusively in the central nervous system (CNS).Functionally, sloβ4 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. It has been shown that co-expression of sloβ4 with KCa1.1 makes the latter resistant to nanomolar concentrations of the well-known inhibitors charybdotoxin and iberiotoxin.The physiological significance of sloβ4 expression in the CNS is not clear, but KCa1.1 channels are likely involved in the regulation of neurotransmitter release in presynaptic terminals.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PC061.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC061; Negative control: BLP-PC061.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
