| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Smad4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Smad4 Rabbit Monoclonal Antibody is an antibody targeting SMAD4. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FH-19; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 70 kDa; calculated MW: 60439 MW.
Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody catalog # M00074. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SMAD4 — Mothers against decapentaplegic homolog 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FH-19; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 70 kDa; Calculated: 60439 MW
Biological background
Protein function (datasheet): In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .
Cellular localization (datasheet): Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta.
Tissue details (datasheet): Ubiquitous. .
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cancer Metabolism,Cardiogenesis,Cardiovascular,Cytoplasmic,Epigenetics and Nuclear Signaling,Hypertrophy,Metabolism,Metabolism Processes,Nuclear Signaling,Nuclear Signaling Pathways,Oncoproteins/Suppressors,Pathways and Processes,Response To Hypoxia,Signal Transduction,Signaling Pathway,Stem Cells,TGF Beta,Transcription Factors,Transcription Factors/Regulators,Tumor Suppressors,Vasculature.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
