| Field | Specification |
|---|---|
| Target | |
| Alternative names | Synaptosomal-associated protein 29; SNAP-29; Soluble 29 kDa NSF attachment protein; Vesicle-membrane fusion protein SNAP-29; SNAP29 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SNAP29 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27S92 is a rabbit monoclonal antibody that recognizes synaptosomal-associated protein 29 (gene SNAP29) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 29.0 kDa, and the supplier reports an observed band at about 29 kDa.
SNAP29 is a SNARE protein that drives fusion of autophagosomes with lysosomes, together with syntaxin-17 and VAMP8. It also takes part in other membrane fusion steps, including those needed for ciliogenesis, and is studied in autophagy research.
| Target | Synaptosomal-associated protein 29 (gene SNAP29; UniProt O95721, human) |
|---|---|
| Clone | 27S92 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.0 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human U2OS cells, human HEL cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human SNAP29.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places synaptosomal-associated protein 29 in the cytoplasm, Golgi apparatus membrane and cell projection. Tissue expression noted by UniProt (human): Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
