| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sorcin;22 kDa protein;CP-22;CP22;V19;SRI; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SR1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sorcin (gene SRI) in human and rat samples and is validated for Western blot. The predicted molecular weight is 21.7 kDa, and the supplier reports an observed band at about 26 kDa.
Sorcin (SRI) is a penta-EF-hand calcium-binding protein that is abundant in heart muscle. It modulates ryanodine receptor RYR2 channels and supports calcium handling in the sarcoplasmic reticulum, so it is studied in cardiac excitation-contraction coupling.
| Target | Sorcin (gene SRI; UniProt P30626, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 21.7 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat liver extract, rat brain extract and SMMC7721 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human SR1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places sorcin in the cytoplasm and sarcoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in cardiac myocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
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