| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Steryl-sulfatase; Arylsulfatase C; ASC; Steroid sulfatase; Steryl-sulfate sulfohydrolase; STS; ARSC1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human STS recombinant protein (Position: K297-Q547). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Steroid sulfatase/STS Antibody Picoband® is an antibody for STS detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: STS (steroid sulfatase); UniProt: P08842
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 65 kDa, calculated 21405 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Steroid sulfatase/STS Antibody Picoband® catalog # A01198-2.
Biological background
Biological context: Conversion of sulfated steroid precursors to estrogens during pregnancy.
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane; Multi-pass membrane protein., tissue context: Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. ..
Common research applications
- Western blotting (WB): Compare STS levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of STS in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify STS-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the metabolism of steroids. It is encoded by the STS gene. This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane; Multi-pass membrane protein.
- Tissue details: Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. .
- Research category: Adapters,Adaptive Immunity,Cell Biology,Immunology,Proteasome / Ubiquitin,Protein Trafficking,Proteolysis/Ubiquitin,Signal Transduction,T Cells,Vesicle Transport
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
