| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromal interaction molecule 1;STIM1;GOK; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Stromal interaction molecule 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HEC-19 is a rabbit monoclonal antibody that recognizes stromal interaction molecule 1 (gene STIM1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 77.4 kDa, and the supplier reports an observed band at about 85 kDa.
STIM1 (stromal interaction molecule 1) is an endoplasmic reticulum Ca2+ sensor. When ER calcium stores are depleted it clusters at ER–plasma membrane junctions and opens ORAI channels, triggering store-operated Ca2+ entry. This pathway is vital for T-cell activation, and STIM1 defects cause immunodeficiency and muscle disorders.
| Target | Stromal interaction molecule 1 (gene STIM1; UniProt Q13586, human) |
|---|---|
| Clone | HEC-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.4 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human HepG2 cells, human HUH-7 cells, rat RH35 cells, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human stromal interaction molecule 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places stromal interaction molecule 1 in the cell membrane, endoplasmic reticulum membrane, cytoplasm and sarcoplasmic reticulum. Tissue expression noted by UniProt (human): Ubiquitously expressed in various human primary cells and tumor cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
