| Field | Specification |
|---|---|
| Target | |
| Alternative names | Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; 1.15.1.1; SOD3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Superoxide Dismutase 3/EC-SOD/SOD3 recombinant protein (Position: E22-A161). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes extracellular superoxide dismutase (gene SOD3) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 25.9 kDa, and the supplier reports an observed band at about 32 kDa.
Extracellular superoxide dismutase (SOD3, EC-SOD) is a secreted copper-zinc enzyme that removes superoxide radicals outside cells, converting them to hydrogen peroxide. By limiting the reaction of superoxide with nitric oxide, it helps preserve nitric oxide signaling in blood vessels, and it is studied in vascular biology, lung injury and inflammation.
| Target | Extracellular superoxide dismutase (gene SOD3; UniProt P08294, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.9 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Superoxide Dismutase 3/EC-SOD/SOD3 fragment (Glu22–Ala161), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes extracellular superoxide dismutase as secreted and also located in the Golgi apparatus and vesicle. Tissue expression noted by UniProt (human): Expressed in blood vessels, heart, lung, kidney and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
