| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pulmonary surfactant-associated protein D;PSP-D;SP-D;Collectin-7;Lung surfactant protein D;SFTPD;COLEC7, PSPD, SFTP4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D, different from the related mouse and rat sequences by eight amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SFTPD (Pulmonary surfactant-associated protein D) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Surfactant protein D/SFTPD Antibody Picoband® catalog # PB9617. Tested in ELISA, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D, different from the related mouse and rat sequences by eight amino acids.
- Molecular weight context: reported MW: 38 kDa; calculated MW: 37728 MW
- Reactivity: Human,Rat
- Applications: ELISA, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Pulmonary surfactant-associated protein D; Pulmonary surfactant-associated protein D. Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. Functional note: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. . Reported localization: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. Expression/tissue context: Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
Research relevance and current trends
- Calcium Binding Proteins: Researchers commonly examine how SFTPD (Pulmonary surfactant-associated protein D) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how SFTPD (Pulmonary surfactant-associated protein D) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how SFTPD (Pulmonary surfactant-associated protein D) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SFTPD (Pulmonary surfactant-associated protein D) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of SFTPD (Pulmonary surfactant-associated protein D) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the SFTPD family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
