| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MRXSYP|Major synaptic vesicle protein p38 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) is an antibody for SYP detection raised in Mouse (Monoclonal, clone Clone: 3G12, Mouse IgG2b), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SYP (synaptophysin); UniProt: Human(P08247), Mouse(Q62277), Rat(P07825)
- Antibody format: Mouse, Monoclonal, clone Clone: 3G12, Mouse IgG2b
- Molecular weight: 38 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) catalog # M05049-3.
Biological background
Biological context: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Expression and localization notes: cellular localization: Cytoplasmic vesicle,secretory vesicle,synaptic vesicle membrane;Multi-pass membrane protein. Cell junction,synapse,synaptosome., tissue context: Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues..
Common research applications
- Western blotting (WB): Compare SYP levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SYP in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SYP-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasmic vesicle,secretory vesicle,synaptic vesicle membrane;Multi-pass membrane protein. Cell junction,synapse,synaptosome.
- Tissue details: Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
- Research category: Cell Adhesion Proteins,Epigenetics and Nuclear Signaling,Integrins,Mediator Complex,Neuroscience,Receptors,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
