| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-box transcription factor TBX21; T-box protein 21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM; TBX21; TBET; TBLYM |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human T-bet/Tbx21, |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-box transcription factor TBX21 in human, mouse and rat samples and is validated for Western blot and flow cytometry.
T-bet (TBX21) is the lineage-defining transcription factor of type 1 helper T (Th1) cells. It turns on genes such as IFN-γ and CXCR3 while repressing Th2 and Th17 programs, and it also drives cytotoxic function in CD8+ T cells and NK cells, making it a standard marker in immunology.
| Target | T-box transcription factor TBX21 (gene TBX21; UniProt Q9UL17, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Immunogen
Synthetic peptide from the middle region of human T-bet/Tbx21.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places T-box transcription factor TBX21 in the nucleus. Tissue expression noted by UniProt (human): T-cell specific.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao-Ming Li et al. (2017) Impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation. American Journal of Physiology-Lung Cellular and Molecular Physiology. 10.1152/ajplung.00409.2016
