| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tafazzin; Protein G4.5; TAZ; EFE2, G4.5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Tafazzin/TAZ recombinant protein (Position: M73-R292). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tafazzin in human samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 33.5 kDa, and the supplier reports an observed band at about 33 kDa.
Tafazzin (TAFAZZIN, TAZ) is a mitochondrial phospholipid transacylase that remodels cardiolipin, exchanging acyl chains between cardiolipin and other phospholipids to give it its mature composition. Correct cardiolipin is needed for respiratory chain complex stability, and loss of tafazzin causes Barth syndrome, a mitochondrial disorder affecting heart and skeletal muscle.
| Target | Tafazzin (gene TAFAZZIN; UniProt Q16635, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 33.5 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Tafazzin/TAZ fragment (Met73–Arg292), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tafazzin in the mitochondrion outer membrane, intermembrane side, mitochondrion inner membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): High levels in cardiac and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haobo Wang et al. (2024) Pulling the Rug Out from Under: Biomechanical Microenvironment Remodeling for Induction of Hepatic Stellate Cell Quiescence. Advanced Materials. 10.1002/adma.202406590
Lan Feng et al. (2023) Dapagliflozin delays renal fibrosis in diabetic kidney disease by inhibiting YAP/TAZ activation. Life Sciences. 10.1016/j.lfs.2023.121671
Baikuntha Aryal et al. (2016) Deficiency in Cardiolipin Reduces Doxorubicin-Induced Oxidative Stress and Mitochondrial Damage in Human B-Lymphocytes. PLoS One. 10.1371/journal.pone.0158376
