| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NADPH oxidase 4;1.6.3.-;Kidney oxidase-1;KOX-1;Kidney superoxide-producing NADPH oxidase;Renal NAD (P)H-oxidase;NOX4;RENOX; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human T-bet / Tbx21 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TBX21 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20T77; isotype IgG; reactivity: Human. Reported application contexts include IHC (as provided in the source record). Boster Bio Anti-TBX21 Rabbit Monoclonal Antibody catalog # M00404-1. Tested in IHC application. This antibody reacts with Human.
Key elements and design rationale
- Target: TBX21 (NADPH oxidase 4).
- Antibody format: Monoclonal; clone 20T77; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TBX21 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. Reported cellular localization context: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus. Tissue expression notes (as provided): Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. .
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,B Cells,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Hematopoietic Progenitors,Immunology,Non-CD,Stem Cells,Surface Molecules,T Cells,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate TBX21 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TBX21 in FFPE tissue sections by immunohistochemistry, Compare relative TBX21 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 52 kDa; calculated MW: 66932 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 52 kDa
- Cellular localization (provided): Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
- Tissue details (provided): Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
