Anti-Tenascin TNN Antibody (Monoclonal, BC-24)

SKU:BHA21006561
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-TNN antibody (Mouse monoclonal, clone BC-24, Mouse IgG1). Validated for Western blot, immunohistochemistry and immunofluorescence. Reactivity: Human. Recognizes tenascin-N. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target TNN
Clone number BC-24
Host Mouse
Reactivity Human
Isotype Mouse IgG1
Application(s) WB, IHC, ICC
Options selector
Catalog no. Size Conjugation
MA1094 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: IHC,ICC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human
      Immunocytochemistry , 1μg/ml, Human, -
      Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, -
      Western blot, 1-2μg/ml, Human
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TNN
Alternative names Protein Tnn ;Tnn ;
Gene ID Human(63923), Mouse(329278), Rat(304913)
UniProt # Q9UQP3
Host Mouse
Clone BC-24
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Human tenascin.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization TNN: Secreted, extracellular space, extracellularmatrix.|Tnn: Secreted, extracellular space, extracellularmatrix.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1094
Main SKU BHA21006561
Mouse Monoclonal

Product Overview

Clone BC-24 is a mouse monoclonal antibody that recognizes tenascin-N (gene TNN) in human samples and is validated for Western blot, IHC and ICC/IF.

Tenascin-N (TNN, also called tenascin-W) is an extracellular matrix glycoprotein of the tenascin family that acts as a ligand for several β1 integrins. It is found in developing bone and other niches, influences neurite outgrowth and cell migration, and is expressed in the stroma of many tumors, where it supports endothelial sprouting and cancer cell motility.

Target Tenascin-N (gene TNN; UniProt Q9UQP3, human)
Clone BC-24
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Human
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–4 µg/mL (Human)
Western blot 1–2 µg/mL (Human)
Immunocytochemistry / Immunofluorescence Not specified by the supplier

Immunogen

Human tenascin.

Reactivity Notes

The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1–2 µg/mL (Human); IHC (paraffin sections) 2–4 µg/mL (Human); Immunocytochemistry / Immunofluorescence (dilution not specified). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Human tenascin.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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