| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Tetraspan NET-3, TSPAN2 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Tetraspanin 2 (extracellular) Antibody is an antibody targeting Tetraspan NET-3, TSPAN2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Tetraspan NET-3, TSPAN2 (also reported as Tetraspan NET-3, TSPAN2).
- Immunogen/epitope region: Extracellular, 2nd loop..
- Homology note: Rat - identical, Human - 14 out of 17 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin", as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR188.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR188; Negative control: BLP-NR188.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
