| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thrombomodulin;TM;Fetomodulin;CD141;THBD;THRM; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Thrombomodulin / CD141 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28T28 is a rabbit monoclonal antibody that recognizes thrombomodulin (gene Thbd) in mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 61.9 kDa, and the supplier reports an observed band at about 105 kDa.
Thrombomodulin (THBD, CD141) is an endothelial surface receptor that binds thrombin and switches it from a procoagulant enzyme to an activator of protein C. The resulting activated protein C pathway limits clotting, and thrombomodulin also affects fibrinolysis, inflammation and angiogenesis. It is studied in vascular biology and thrombosis, and CD141 marks a subset of human dendritic cells.
| Target | Thrombomodulin (gene Thbd; UniProt P15306, mouse) |
|---|---|
| Clone | 28T28 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.9 kDa |
| Observed MW | 105 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): mouse lung cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human thrombomodulin / CD141.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places thrombomodulin in the membrane. Tissue expression noted by UniProt (mouse): Endothelial cells are unique in synthesizing thrombomodulin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
