| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1; CD antigen CD365; HAVCR1; KIM1; TIM1; TIMD1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TIM1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFBC-8 is a rabbit monoclonal antibody that recognizes hepatitis A virus cellular receptor 1 (gene HAVCR1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 39.3 kDa, and the supplier reports an observed band at about 50 kDa.
HAVCR1 (TIM-1, KIM-1) is a transmembrane phosphatidylserine receptor of the TIM family. On injured kidney tubule cells, it is strongly induced and enables them to engulf apoptotic cells, and its shed ectodomain is a widely studied marker of kidney injury. TIM-1 also regulates immune cells, including regulatory B cells, and serves as an entry factor for some viruses.
| Target | Hepatitis A virus cellular receptor 1 (gene HAVCR1; UniProt Q96D42, human) |
|---|---|
| Clone | AFBC-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.3 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TIM1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hepatitis A virus cellular receptor 1 in the cell membrane, cell projection and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in kidney and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Meng Zhe et al. (2013) Suppression of renal TRPM7 may alleviate kidney injury in the renal transplantation. World Journal of Urology. 10.1007/s00345-013-1208-y
Songsong Wang et al. (2021) The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/5566078
