| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 3; CD283; TLR3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TLR3 recombinant protein (Position: F502-D648). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects toll-like receptor 3 (gene TLR3) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 103.8 kDa, and the supplier reports an observed band at about 120 kDa.
Toll-like receptor 3 (TLR3) is an endosomal pattern recognition receptor that senses double-stranded RNA, a hallmark of many viral infections. Unlike most TLRs it signals through the adaptor TRIF rather than MyD88, driving IRF3 and NF-κB activation and type I interferon production. TLR3 is studied in antiviral immunity and with the synthetic agonist poly(I:C).
| Target | Toll-like receptor 3 (gene TLR3; UniProt O15455, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 103.8 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, mouse RAW264.7 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TLR3 fragment (Phe502–Asp648), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places toll-like receptor 3 in the endoplasmic reticulum membrane, endosome membrane and early endosome. Tissue expression noted by UniProt (human): Expressed at high level in placenta and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dengjin Chen et al. (2022) Astragalus polysaccharide alleviated the inhibition of CSFV C-strain replication caused by PRRSV via the TLRs/NF‑κB/TNF-α pathways. Virus Research. 10.1016/j.virusres.2022.198854
Liejun Liao et al. (2021) Bioinformatic and biochemical studies of formononetin against liver injure. Life Sciences. 10.1016/j.lfs.2021.119229
Yuxi Li et al. (2019) A Study of the Immunoregulatory Function of TLR3 and TLR4 on Mesenchymal Stem Cells in Ankylosing Spondylitis. Stem Cells and Development. 10.1089/scd.2019.0039
