| Field | Specification |
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| Mfr No | |
| Accession Number | |
| Alternative Names | Transmembrane Protein 119, Osteoblast induction factor, OBIF |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
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Overview
Anti-TMEM119 (extracellular)-PE Antibody is an antibody targeting Transmembrane Protein 119, Osteoblast induction factor, OBIF Polyclonal raised in Rabbit (R-Phycoerythrin (R-PE)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF (also reported as Transmembrane Protein 119, Osteoblast induction factor, OBIF).
- Immunogen/epitope region: Extracellular, N-term..
- Homology note: Rat – 12 out of 15 amino acid residues identical. This antibody will not recognize human TMEM119 (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ANR-175), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: R-Phycoerythrin (R-PE) (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1. Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-PE.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR175; Negative control: RIC-001-PE (name: Rabbit IgG Isotype Control-PE).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
