| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-TMEM175 Antibody is an antibody targeting Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175 (also reported as Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175).
- Immunogen/epitope region: Luminal, 4th loop..
- Homology note: Rat - 13 out of 14 amino acid residues identical Human - 12 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Transmembrane protein 175, TMEM175, is an endosomal/lysosomal proton channel that plays a crucial role in preserving the lysosomal membrane potential and maintaining pH stability. TMEM175 mediates lysosomal proton efflux, known as the "H+ leak" pathway, preventing excessive acidification of lysosomes.1 Intracellular endosomes and lysosomes rely on a vacuolar-type H+ ATPase (V-ATPase) to establish a significant proton concentration gradient across their membranes, which plays a crucial role in facilitating active transport of ions and metabolites between the vesicular lumen and the cytosol. To effectively digest macromolecules, lysosomes rely on an acidic environment with a pH range of 4.5 to 5.0, which is maintained through the entry of protons by the V-ATPase pump and their exit through the "H+ leak" pathway, which is the passive release of H+ into the cytosol.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PC175.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC175; Negative control: BLP-PC175.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
