| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Topoisomerase II alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FB-20 is a rabbit monoclonal antibody that recognizes DNA topoisomerase 2-alpha (gene TOP2A) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 174.4 kDa, and the supplier reports an observed band at about 174 kDa.
DNA topoisomerase II alpha (TOP2A) untangles DNA by cutting both strands of one duplex, passing another duplex through the gap and resealing the break. This decatenation is needed to separate sister chromatids and condense chromosomes in mitosis. TOP2A is highly expressed in proliferating cells, serves as a proliferation marker, and is the target of etoposide and anthracyclines.
| Target | DNA topoisomerase 2-alpha (gene TOP2A; UniProt P11388, human) |
|---|---|
| Clone | FB-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 174.4 kDa |
| Observed MW | 174 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human topoisomerase II alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA topoisomerase 2-alpha in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
