| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein 75 kDa, mitochondrial; HSP 75; Heat shock protein family C member 5; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1; TRAP1; HSP75; HSPC5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRAP1 recombinant protein (Position: A618-H704). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects heat shock protein 75 kDa, mitochondrial (gene TRAP1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 80.1 kDa, and the supplier reports an observed band at about 80 kDa.
TRAP1 (heat shock protein 75, mitochondrial) is the mitochondrial member of the HSP90 chaperone family. It helps maintain mitochondrial protein folding and membrane potential and acts as a brake on oxidative phosphorylation, shifting the balance toward glycolysis. TRAP1 is often elevated in tumors and is studied in cancer metabolism and mitochondrial stress responses.
| Target | Heat shock protein 75 kDa, mitochondrial (gene TRAP1; UniProt Q12931, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 80.1 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HL-60 cells, human 293T cells, human U-87MG cells, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human TRAP1 fragment (Ala618–His704), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heat shock protein 75 kDa, mitochondrial in the mitochondrion, mitochondrion inner membrane and mitochondrion matrix. Tissue expression noted by UniProt (human): Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
