| Field | Specification |
|---|---|
| Target | |
| Alternative names | High affinity nerve growth factor receptor |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TrkA, which shares 90.2% amino acid (aa) sequence identity with both mouse and rat TrkA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes high affinity nerve growth factor receptor (gene NTRK1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 87.5 kDa, and the supplier reports an observed band at about 150 kDa.
TrkA (NTRK1) is the high-affinity receptor tyrosine kinase for nerve growth factor (NGF). NGF binding triggers receptor autophosphorylation and activation of MAPK, PI3K/AKT and PLCγ signaling that supports the survival and differentiation of sensory and sympathetic neurons, and TrkA is studied in pain, neurodevelopment and NTRK fusion-driven cancers.
| Target | High affinity nerve growth factor receptor (gene NTRK1; UniProt P04629, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 87.5 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human HeLa cells, human SGC-7901 cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human trkA. Sequence identity with the mouse and rat orthologs: 90.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places high affinity nerve growth factor receptor in the cell membrane, early endosome membrane, late endosome membrane and recycling endosome membrane. Tissue expression noted by UniProt (human): Isoform TrkA-I is found in most non-neuronal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
