Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31)

SKU:BHA21006562
Research Validated
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Overview
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Anti-TPM1 antibody (Mouse monoclonal, clone TM31, Mouse IgG1). Validated for Western blot. Reactivity: Chicken, Human, Mouse, Rat. Recognizes tropomyosin alpha-1 chain. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target TPM1
Clone number TM31
Host Mouse
Reactivity Chicken,Human,Mouse,Rat
Isotype Mouse IgG1
Application(s) WB
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Options selector
Catalog no. Size Conjugation
MA1095 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: WB
      Application details: Western blot, 0.5-1μg/ml, Human, mouse, rat, chicken
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TPM1
Alternative names Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1;Tpm1;Alpha-tm, Tpma;
Gene ID Human(7168), Mouse(22003), Rat(24851)
UniProt # P04692
Host Mouse
Clone TM31
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen Chicken gizzard tropomyosin.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization Cytoplasm, cytoskeleton.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1095
Main SKU BHA21006562
Mouse Monoclonal

Product Overview

Clone TM31 is a mouse monoclonal antibody that recognizes tropomyosin alpha-1 chain (gene Tpm1) in chicken, human, mouse and rat samples and is validated for Western blot.

Tropomyosin alpha-1 (TPM1) is a coiled-coil protein that lies along actin filaments. In striated muscle, it works with the troponin complex to control calcium-dependent contraction, and in smooth muscle and non-muscle cells it stabilizes actin filaments and shapes the cytoskeleton. TPM1 mutations are associated with inherited cardiomyopathies.

Target Tropomyosin alpha-1 chain (gene Tpm1; UniProt P04692, rat)
Clone TM31
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Chicken, Human, Mouse, Rat
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.5–1 µg/mL (Human, Mouse, Rat, Chicken)

Immunogen

Chicken gizzard tropomyosin.

Reactivity Notes

The supplier lists reactivity with chicken, human, mouse and rat. UniProt places tropomyosin alpha-1 chain in the cytoplasm.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.5–1 µg/mL (Human, Mouse, Rat, Chicken). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Chicken, Human, Mouse, Rat. Other species are not listed by the supplier.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What immunogen was used to raise this antibody?
A.Chicken gizzard tropomyosin.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Cheng Lijun et al. (2021) Proteomic Sequencing of Stellate Ganglions in Rabbits With Myocardial Infarction. Frontiers in Physiology. 10.3389/fphys.2021.687424

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