| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1;Tpm1;Alpha-tm, Tpma; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Chicken gizzard tropomyosin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) is an antibody targeting TPM1. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: TM31; isotype: Mouse IgG1; reactivity: Chicken,Human,Mouse,Rat; observed MW: 35-45 kDa; calculated MW: 32681 MW.
Boster Bio Anti-Tropomyosin (36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) catalog # MA1095. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Key elements and design rationale
- Target: TPM1 — Tropomyosin alpha-1 chain
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: TM31; Isotype: Mouse IgG1
- Species reactivity: Chicken,Human,Mouse,Rat
- Molecular weight guidance: Observed: 35-45 kDa; Calculated: 32681 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Scientific background (datasheet): The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
Cellular localization (datasheet): Cytoplasm, cytoskeleton.
Tissue details (datasheet): TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.
Sequence similarities (datasheet): Belongs to the tenascin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin, etc.,Cardiovascular,Cytoskeleton,Cytoskeleton/ECM,Hypertrophy,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
