| Field | Specification |
|---|---|
| Target | |
| Alternative names | Short transient receptor potential channel 3;TrpC3;Transient receptor protein 3;TRP-3;hTrp-3;hTrp3;TRPC3;TRP3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRPC3 recombinant protein (Position: R718-L770). Human TRPC3 shares 96.2% amino acid (aa) sequence identity with both mouse and rat TRPC3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes short transient receptor potential channel 3 (gene TRPC3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 96.0 kDa, and the supplier reports an observed band at about 106 kDa.
TRPC3 is a canonical transient receptor potential channel that opens after activation of G protein-coupled receptors or receptor tyrosine kinases, which stimulate phospholipase C. It allows non-selective cation and calcium entry, can be activated by diacylglycerol, and is studied in neurons, cardiac and smooth muscle and in calcium signaling.
| Target | Short transient receptor potential channel 3 (gene TRPC3; UniProt Q13507, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 96.0 kDa |
| Observed MW | 106 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): human HEL cells, human U2OS cells, human 293T cells, human U251 cells, rat C6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TRPC3 fragment (Arg718–Leu770), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places short transient receptor potential channel 3 in the cell membrane. Tissue expression noted by UniProt (human): Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Yu et al. (2020) TRPC6 participates in the development of blood pressure variability increase in sino-aortic denervated rats. Heart and Vessels. 10.1007/s00380-020-01682-1
