| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TSG101 recombinant protein (Position: E223-K257). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor susceptibility gene 101 protein (gene TSG101) in human, mouse and rat samples and is validated for Western blot, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 43.9 kDa, and the supplier reports an observed band at about 44 kDa.
TSG101 is a core subunit of ESCRT-I, which binds ubiquitinated membrane proteins and sorts them into multivesicular bodies. It also acts in cytokinesis and is used by many enveloped viruses, including HIV-1, whose Gag protein recruits TSG101 through a PTAP motif to bud from cells. TSG101 is widely used as a marker of exosomes.
| Target | Tumor susceptibility gene 101 protein (gene TSG101; UniProt Q99816, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 43.9 kDa |
| Observed MW | 44 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human Colo320 cells, human Sw620 cells, human Caco-2 cells, human Hek293 cells, human K562 cells, human HepG2 cells, human THP-1 cells, rat brain tissue, rat testis tissue, rat liver tissue, rat spleen tissue, mouse brain tissue, mouse testis tissue, mouse liver tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed result (IP): HepG2 cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human TSG101 fragment (Glu223–Lys257), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tumor susceptibility gene 101 protein in the cytoplasm, early endosome membrane and late endosome membrane. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-yan Yan et al. (2025) Low miR-224-5p in exosomes confers colorectal cancer 5-FU resistance by upregulating S100A4. Drug Resistance Updates. 10.1016/j.drup.2025.101211
Zhen Chen et al. (2024) Engineering Macrophage-Derived Exosome to Deliver Pirfenidone: A Novel Approach to Combat Silicotic Pulmonary Fibrosis. Advanced Healthcare Materials. 10.1002/adhm.202403227
Liuwan Zhao et al. (2019) Exosome-mediated siRNA delivery to suppress postoperative breast cancer metastasis. Journal of Controlled Release. 10.1016/j.jconrel.2019.12.005
Jingwei Yu et al. (2023) Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes. Biochemical Pharmacology. 10.1016/j.bcp.2023.115462
Cong Ma et al. (2025) Mir-574-3p and Mir-125a-5p in Adipose Derived Mesenchymal Stem Cell Exosomes Synergistically Target TGF-β1/Smad2 Signaling Pathway for the Treatment of Androgenic Alopecia. Journal of Investigative Dermatology. 10.1016/j.jid.2025.03.042
Hao Zhang et al. (2025) Heme oxygenase 1‑overexpressing bone marrow mesenchymal stem cell‑derived exosomes suppress interleukin‑1 beta‑induced apoptosis and aging of nucleus pulposus cells. Molecular Medicine Reports. 10.3892/mmr.2025.13481
