| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor susceptibility gene 101 protein;ESCRT-I complex subunit TSG101;TSG101; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TSG101 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IDG-20 is a rabbit monoclonal antibody that recognizes tumor susceptibility gene 101 protein (gene TSG101) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 43.9 kDa, and the supplier reports an observed band at about 44 kDa.
TSG101 is a core subunit of ESCRT-I, which binds ubiquitinated membrane proteins and sorts them into multivesicular bodies. It also acts in cytokinesis and is used by many enveloped viruses, including HIV-1, whose Gag protein recruits TSG101 through a PTAP motif to bud from cells. TSG101 is widely used as a marker of exosomes.
| Target | Tumor susceptibility gene 101 protein (gene TSG101; UniProt Q99816, human) |
|---|---|
| Clone | IDG-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.9 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Jurkat cells, human COLO320 cells, human SW620 cells, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue, rat liver tissue, mouse kidney tissue, rat stomach tissue, human tongue cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human TSG101.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tumor susceptibility gene 101 protein in the cytoplasm, early endosome membrane and late endosome membrane. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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