| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tetraspanin-12; Tspan-12; Tetraspan NET-2; Transmembrane 4 superfamily member 12; TSPAN12; NET2; TM4SF12; UNQ774/PRO1568 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TSPAN12 recombinant protein (Position: G111-R224). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tetraspanin-12 (gene TSPAN12) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 35.4 kDa, and the supplier reports an observed band at about 40 kDa.
Tetraspanin-12 (TSPAN12) is a membrane protein that works with norrin, the receptor FZD4 and LRP5 to activate β-catenin signaling that drives vascular development of the retina. Mutations cause familial exudative vitreoretinopathy, and TSPAN12 has also been reported to regulate membrane proteases such as ADAM10 and MMP14.
| Target | Tetraspanin-12 (gene TSPAN12; UniProt O95859, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 35.4 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Immunohistochemistry | Not specified by the supplier |
Samples with a confirmed band (WB): human HepG2 cells, rat kidney tissue, rat NRK cells, mouse kidney tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human TSPAN12 fragment (Gly111–Arg224), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tetraspanin-12 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
